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Visualizing RNA Localization in Xenopus Oocytes
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Biologia
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JoVE Journal Biologia
Visualizing RNA Localization in Xenopus Oocytes

Visualizing RNA Localization in Xenopus Oocytes

DOI:
10.3791/1704-v

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07:06 min

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January 14, 2010

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1Department of Molecular Biology, Cell Biology, and Biochemistry,Brown University

Capitoli

  • 00:00Titolo
  • 00:30Introduction
  • 01:03Preparing for Microinjection
  • 03:14Microinjection and Oocyte Culture
  • 04:35Fixation, Dehydration, and Storage of Oocytes
  • 05:35Imaging RNA Localization by Confocal Microscopy
  • 05:59RNA Localization in Xenopus Oocytes: Representative Results
  • 06:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.

Tags

RNA LocalizationXenopus OocytesCell PolarityVg1 MRNAGene ExpressionGerm Layer SpecificationRNA Sequence ElementsVg1 Localization Element (VLE)Trans-factor Directed TransportImaging TechniqueFluorescently Labeled VLE RNAMicroinjectionConfocal MicroscopyMRNA Localization PatternsCis-acting ElementsTrans-acting FactorsCo-localizationMotor ProteinsCytoskeleton

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