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Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
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Biologia
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JoVE Journal Biologia
Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis

DOI:
10.3791/1707-v

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07:29 min

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January 08, 2010

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, , , ,
1Monash Immunology and Stem Cell Laboratories,Monash University, 2Anatomy and Developmental Biology,Monash University

Capitoli

  • 00:00Titolo
  • 01:06Specimen Preparation and Fixation
  • 06:50Conclusion

Summary

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Undecalcified bone histology provides important information for a variety of clinical and research applications. It is technically challenging, particularly with large size specimens. This video illustrates the process of producing good quality sections and demonstrates the technical difficulties and methods with which to overcome them.

Tags

Undecalcified Bone PreparationHistologyHistomorphometryFluorochrome AnalysisBone TurnoverBone FormationBone ResorptionClinical ApplicationsResearch ApplicationsBeautiful ImagesFluorochrome AssessmentMicroscopic LevelTechnically ChallengingLarge Size SpecimensFixation And ProcessingEmbedding

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