Rivista
/
Biologia
/
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
JoVE Journal
Biologia
Author Produced
This content is Free Access.
JoVE Journal Biologia
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

DOI:
10.3791/1712-v

•

22:00 min

•

November 21, 2010

•

,
1Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine,Trinity College Dublin

Capitoli

  • 00:00Introduction
  • 02:48Materials
  • 03:48Preparing the Crystallization Plate
  • 05:12Preparing the Lipid and Protein
  • 13:53Loading the Dispensing Syringe
  • 17:57Dispensing
  • 21:10Crystals

Summary

Traduzione automatica

Traduzione automatica

Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.

Tags

Crystallizing Membrane ProteinsLipidic MesophasesLipidic Cubic PhaseIn Meso MethodX-ray Crystallographic StructuresProkaryotic ProteinsEukaryotic ProteinsMonomeric ProteinsHomo-multimeric ProteinsHetero-multimeric ProteinsChromophore-containing ProteinsChromophore-free ProteinsAlpha-helical ProteinsBeta-barrel ProteinsBeta2-adrenergic ReceptorAdenosine A2a ReceptorReconstituting Membrane ProteinMonoolein-based MesophaseManual Crystallization SetupCrystal Harvesting

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/1712?article_type=v

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

check_url/it/1712?article_type=v

Crystallization of Membrane Proteins in Lipidic Mesophases
check_url/it/1712?article_type=v

Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
check_url/it/1712?article_type=v

High-throughput krystallisering af membranproteiner Brug af Lipidic Bicelle Metode
check_url/it/1712?article_type=v

Anvendelse af en robot til Højkapacitetsforskning Krystallisation af membranproteiner i Lipidiske mesofaser
check_url/it/1712?article_type=v

Høst og Cryo-køling Krystaller af membranproteiner dyrket i Lipidiske mesofaser for Structure Bestemmelse ved makromolekylær krystallografi
check_url/it/1712?article_type=v

Cellesporing hjælp Photoconvertible proteiner under zebrafisk Development
check_url/it/1712?article_type=v

RNA Secondary Structure Prediction Using High-throughput SHAPE
check_url/it/1712?article_type=v

Intravital mikroskopi af Imaging Subcellulære Strukturer Live mus, der udtrykker fluorescerende proteiner
check_url/it/1712?article_type=v

Grønt fluorescerende protein-baserede Expression Screening membranproteiner i<em> Escherichia coli</em

Read Article