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Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
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Biologia
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JoVE Journal Biologia
Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

DOI:
10.3791/1714-v

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05:29 min

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February 11, 2010

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,
1Department of Molecular and Cell Biology,University of Connecticut

Capitoli

  • 00:00Titolo
  • 00:37Introduction
  • 01:27Hemolymph Extraction from Euprymna scolopes
  • 02:43Hemocyte Preparation and Staining
  • 04:23Visualization of Hemocytes and Associated Bacteria
  • 04:56Conclusion

Summary

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This video will demonstrate how to obtain hemocytes (blood cells) from the Hawaiian bobtail squid, Euprymna scolopes for use in cell biological and bacterial adhesion assays. Hemocytes will be stained with a fluorescent dye and exposed to GFP-labeled bacteria.

Tags

HemocytesHawaiian Bobtail SquidEuprymna ScolopesAdherenceSymbiotic BacteriaNon-symbiotic BacteriaImmune SystemMolecular SignalingBeneficial BacteriaCo-evolutionEukaryotesMicrobiotaModel SystemAnimal DevelopmentMacrophage-like HemocytesInnate Immune SystemEstablishmentMaintenanceProtocolAnesthetizedHemolymph CollectionCephalic Blood VesselChambered Glass CoverslipsGreen Fluorescent Protein-labeled Symbiotic Vibrio FischeriNon-symbiotic Vibrio HarveyiFluorescent DyeCell Tracker OrangeVisualizedFluorescent Microscopy

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