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Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
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Biologia
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JoVE Journal Biologia
Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

DOI:
10.3791/1722-v

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07:23 min

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July 16, 2010

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, ,
1Department of Cell and Developmental Biology,University of Pennsylvania

Capitoli

  • 00:11Introduction
  • 00:42ID of Candidate Seq & Expression Plasmid Construction
  • 02:24Microinjection of Zebrafish Embryos
  • 05:50Analysis of Injected Embryos
  • 05:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

We demonstrate our approach to finding potential enhancer elements from developmentally regulated genes and evaluating their function through mosaic zebrafish transgenesis.

Tags

Mosaic Zebrafish TransgenesisEnhancer SequencesHuman Genome SequenceNon Coding SequencesGene TranscriptionCis-regulatory ElementsZebrafish TransgenesisCell-culture Based TechniquesSpatial And Temporal Gene RegulationTransgenic MiceHuman GenomeSequence ConservationCloning SequencesMicroinjectingZebrafish Embryos

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