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Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
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Biologia
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JoVE Journal Biologia
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

DOI:
10.3791/1726-v

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10:52 min

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February 03, 2010

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, , ,
1Genetics Training Program,University of Wisconsin-Madison, 2Department of Anatomy,University of Wisconsin-Madison, 3Department of Zoology,University of Wisconsin-Madison, 4Cell and Molecular Biology Training Program,University of Wisconsin-Madison

Capitoli

  • 00:00Titolo
  • 00:47Introduction
  • 01:18Assembling Injection Slides and Imaging Slides
  • 02:36Injecting DNA Constructs
  • 04:43Mounting Embryos for Imaging
  • 06:524D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
  • 08:49Behavior of Individual Labeled Neurons and Neural Crest Cells
  • 10:23Conclusion

Summary

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This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.

Tags

Live ImagingCell MotilityActin CytoskeletonIndividual NeuronsNeural Crest CellsZebrafish EmbryosDevelopmentIn VivoOptical ClarityHigh Resolution ImagingCell BehaviorsMigrationAxon OutgrowthGuidanceMechanismsCell LabelingPlasmid DNA InjectionTransient Mosaic Expression PatternProtrusive ActivityMolecular DynamicsTransgenic LinesFine ProtrusionsChanges In Molecular Distribution

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