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Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
DOI:

11:26 min

May 05, 2010

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Capitoli

  • 00:00Titolo
  • 00:45Introduction
  • 01:34Gene Introduction Using Particle Bombardment
  • 04:51Automated Image Collection
  • 07:44Image Analysis
  • 09:14Representative Results of Gene Expression Imaging in Plant Tissues
  • 10:12Conclusion

Summary

Traduzione automatica

We report a method for introduction, tracking and quantitative analysis of GFP expression in plant cells. This method utilizes a custom-designed robotics system for semi-continuous image collection from large numbers of samples, over time. We also demonstrate the use of ImageJ and ImageReady for analysis of image series.

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