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A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
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Biologia
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JoVE Journal Biologia
A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

DOI:
10.3791/1779-v

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09:28 min

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April 19, 2010

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, ,
1McFerrin Department of Chemical Engineering,Texas A&M University, 2Department of Biology,Texas A&M University, 3Department of Biomedical Engineering,Texas A&M University

Capitoli

  • 00:00Titolo
  • 00:31Introduction
  • 00:57Replica Molding of PDMS from SU-8 Master
  • 02:02Bonding and Assembling the PDMS Device
  • 03:40Growing Highly Motile E.coli
  • 04:49Monitoring Chemotaxis
  • 05:52Data Analysis
  • 07:51Representative Chemotaxis Results
  • 08:59Conclusion

Summary

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This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

Tags

Microfluidic DeviceQuantifying Bacterial ChemotaxisConcentration GradientsChemoeffectorsMigrationAttractantsRepellentsMicrofluidic Chemotaxis ModelPrecise And Stable Concentration GradientsBacteria ResponseEscherichia Coli RP437Amino AcidsNickel Ions

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