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Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
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JoVE Journal Biologia
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

DOI:
10.3791/183-v

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09:19 min

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April 28, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI)

Capitoli

  • 00:08Introduction
  • 00:52Preparation of umbilical cord
  • 02:00Digestion of umbilical vein and isolation of HUVEC

Summary

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This video protocol illustrates the isolation and culture of human umbilical vein endothelial cells (HUVEC) from human umbilical cord. Once isolated these cells can be used for in vitro angiogenesis assays like the Optimized Fibrin Gel Bead Assay also demonstrated by the Hughes lab.

Tags

IsolationHuman Umbilical Vein Endothelial CellsHUVECAngiogenesisMulti-step ProcessVascular DegradationProliferationMigrationSproutingExtracellular MatrixAlignmentCord FormationLumen FormationAnastomosisNew Basement MembraneIn Vitro AssaysMorphological ResemblanceOptimized In Vitro Angiogenesis AssayHuman Umbilical Vein EC And FibroblastsEarly Stages Of AngiogenesisIntercellular LumensPolarized ECPhase-contrast MicroscopyTime-lapse MicroscopyDownstream Applications

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