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Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
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Biologia
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JoVE Journal Biologia
Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

DOI:
10.3791/1852-v

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08:18 min

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March 22, 2010

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,
1Department of Chemical Engineering and Materials Science,City of Hope Cancer Center

Capitoli

  • 00:00Titolo
  • 00:47Introduction
  • 01:18Isolation of Rat MSCs
  • 02:40Enrichment of Rat MSCs
  • 05:17Separation of Single-colony Derived MSCs
  • 06:36Results of Isolation and Enrichment of Rat MSCs
  • 07:41Conclusion

Summary

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Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.

Tags

IsolationEnrichmentRat Mesenchymal Stem CellsMSCsSingle-colony Derived MSCsBone MarrowHematopoietic Stem CellsPlastic AdherenceProtocolRat FemursTibiasMagnetic Cell SortingSurface MarkersCD54CD90Flow Cytometry AnalysisHSC Marker CD45HeterogeneitySubpopulationsCloning CylindersCulture Evaluation

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