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在甲醇酵母表达的重组蛋白毕赤酵母</em
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Biologia
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JoVE Journal Biologia
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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在甲醇酵母表达的重组蛋白毕赤酵母</em

DOI:
10.3791/1862-v

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09:46 min

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February 25, 2010

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, ,
1Department of Microbiology and Immunology,University of British Columbia – UBC

Capitoli

  • 00:00Titolo
  • 00:31Introduction
  • 01:05Preparing the pPICZaA Construct
  • 02:18Preparation of Electrocompetent Yeast Cells
  • 04:04Transformation by Electroporation
  • 06:06Protein Expression in Pichia pastoris
  • 08:42Results of Recombinant Protein Expression
  • 09:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

协议描述使用甲醇酵母蛋白的表达<em>毕赤酵母</em>。 electrocompetent酵母细胞的准备，与感兴趣的基因到改造的载体<em> P。酵母</em>和酵母DNA纯化。 Western blot分析和蛋白质纯化，建立在这种蛋白的表达协议的最后步骤。

Tags

Recombinant ProteinsPichia PastorisProtein ExpressionMethylotrophic YeastExpression SystemPost-translational ModificationsProtein FoldingProteolytic ProcessingDisulfide Bond FormationGlycosylationMethanol MetabolismAlcohol Oxidase PromoterMethanol InductionSecretion Of Recombinant ProteinSignal SequenceHeterologous ProteinProtein PurificationVector

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