È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:07Introduction
- 01:15Tools and materials
- 02:23Dissection of organizer
- 05:49Dissection of animal pole
- 06:52Dissociation of organizer
- 09:28Constructing imaging chamber
- 11:57Analysis
- 13:44Conclusion
This video demonstrates the technique used for preparation of organizer and animal pole explants from Xenopus laevis embryos, including the use of the eyebrow knife - a specialized dissection tool made of one's eyebrow. The protocol for assembling an adhesion assay is also given, which probes for the presence of key adhesion molecules present on the surface organizer or animal pole cells that are critical for proper development.
