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Organotypic Culture of Adult Rabbit Retina
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Biologia
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JoVE Journal Biologia
Organotypic Culture of Adult Rabbit Retina

Organotypic Culture of Adult Rabbit Retina

DOI:
10.3791/190-v

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10:12 min

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April 29, 2007

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, , ,
1Havard Medical School,MGH – Massachusetts General Hospital

Capitoli

  • 00:14Preparation of retina
  • 05:03Gene-gun transfection and incubation of retina
  • 06:37Discussion

Summary

Traduzione automatica

Traduzione automatica

This article demonstrates the dissection and incubation of rabbit retina and particle-mediated gene transfer of plasmids encoding GFP or a variety of subcellular markers into retinal ganglion cells.

Tags

Organotypic CultureAdult Rabbit RetinaNeurobiological ResearchImaging TechniquesGenetic ManipulationElectrophysiological RecordingSpecialized EquipmentMaintenanceDissectionIncubationGene TransferPlasmids Encoding GFPSubcellular MarkersRetinal Ganglion CellsAnatomical StructureMorphology

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