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Capitoli
- 00:00Titolo
- 01:16Introduction
- 01:39Preparation of the Halted RNAP Elongation Complex
- 03:07Preparation of the Downstream Replicaton Fork
- 04:06Assembly of the Replisome and Initiation of Leading Strand Synthesis at the Replication Fork
- 05:49Representative Results for the Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
- 06:49Conclusion
The fate of the replisome following a collision with a head-on RNA polymerase (RNAP) is unknown. We find that the replisome stalls upon collision with a head-on RNAP, but resumes elongation after displacing the RNAP from DNA. Mfd promotes replication restart by facilitating displacement of the RNAP after the collision.
