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发展和成人的制备果蝇大脑和视网膜实时成像
JoVE Journal
Biologia
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JoVE Journal Biologia
Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
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发展和成人的制备果蝇大脑和视网膜实时成像

DOI:
10.3791/1936-v

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16:47 min

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March 15, 2010

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1Department of Physiology and Green Center for Systems Biology,University of Texas Southwestern Medical Center

Capitoli

  • 00:00Titolo
  • 00:07Introduction
  • 00:49Dissection Preparation
  • 03:21Adult Brain Dissection
  • 07:25Adult Eye Dissection
  • 11:14Developing Eye Disk-brain Complexes
  • 12:30Live Imaging: at the microscope
  • 15:45Conclusion
  • 16:06Representative Results: live imaging

Summary

Traduzione automatica

Traduzione automatica

本协议描述了三个<em>果蝇</em>准备工作：1）成人大脑清扫，2）成人视网膜剥离和3）发展中国家眼睛光盘大脑复合物清扫。重点是放在特殊的制备技术和条件，实时成像，可用于固定组织的免疫组织化学，但所有的准备工作。

Tags

Drosophila BrainRetinaLive ImagingNeuronal DevelopmentFunctionDegenerationEye Disc-brain ComplexPupaeAdult FliesLive CultureConfocal Live ImagingPerfusion ChamberTime Scales

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