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Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus
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Biologia
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JoVE Journal Biologia
Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

DOI:
10.3791/1944-v

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09:16 min

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April 09, 2010

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, ,
1Department of Chemical and Life Sciences Engineering,Virginia Commonwealth University

Capitoli

  • 00:00Titlle
  • 00:12Introduction
  • 08:26Representative Results
  • 08:42Conclusion
  • 09:02Credits

Summary

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Suspension immunocytochemical staining of human pluripotent stem cells (hPSCs) for cell-surface markers (SSEA-3/SSEA-4) was achieved based on use of a self-made cytospin apparatus to create a monolayer of cells for observation and quantification.

Tags

Immunocytochemical AnalysisHuman Pluripotent Stem CellsHPSCsHuman Embryonic Stem CellsHESCsHuman Induced Pluripotent Stem CellsHiPSCsSelf-made Cytospin ApparatusCell SourcesSelf-renewal CapabilitiesDifferentiationQPCRIn Vitro DifferentiationIn Vivo DifferentiationIntracellular BiomarkersCell-surface BiomarkersColony GrowthMarker EvaluationMonolayer Cell Samples

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