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Quantification of γH2AX Foci in Response to Ionising Radiation
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Biologia
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JoVE Journal Biologia
Quantification of γH2AX Foci in Response to Ionising Radiation

Quantification of γH2AX Foci in Response to Ionising Radiation

DOI:
10.3791/1957-v

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06:53 min

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April 06, 2010

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, , , , ,
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Department of Pathology,The University of Melbourne, 3Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct

Capitoli

  • 00:00Titolo
  • 00:06Introduction
  • 01:25Irradiation
  • 06:01Analysis
  • 06:32Conclusion

Summary

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Quantification of DNA double-strand streaks using γH2AX formation as a molecular marker has become an invaluable tool in radiation biology. Here we demonstrate the use of an immunofluorescence assay for quantification of γH2AX foci after exposure of cells to radiation.

Tags

γH2AX FociQuantificationIonising RadiationDNA Double-strand BreaksH2A Histone VariantPhosphorylationSerine-139 ResidueC-terminal SQEY MotifPhosphatidyl-inosito 3-kinase (PIKK) FamilyAtaxia Telangiectasia Mutated (ATM)DNA-protein Kinase Catalytic SubunitATM And RAD3-related (ATR)Chromatin ModificationsDNA Damage Signalling And RepairImmunofluorescence MicroscopyRepair Of DSBs

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