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Murine Bioluminescent Hepatic Tumour Model
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JoVE Journal Medicina
Murine Bioluminescent Hepatic Tumour Model

Murine Bioluminescent Hepatic Tumour Model

DOI:
10.3791/1977-v

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05:23 min

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July 17, 2010

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, , , , , ,
1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory,University College Cork, 2Department of Computer Science,University College Cork, 3South Infirmary Victoria University Hospital

Capitoli

  • 00:00Titolo
  • 00:05Introduction
  • 01:24Materials
  • 02:12Preparation of the Mouse
  • 02:49Abdominal Incision
  • 02:58Exposureand Division of Spleen
  • 03:17Injection of Cells into One Hemi-Spleen
  • 03:50Excision of Injection Hemi-Spleen
  • 03:55Abdominal Closure
  • 04:13Whole Body Imaging

Summary

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This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.

Tags

MurineBioluminescentHepatic Tumour ModelLiver MetastasesMouse ModelBioluminescent ImagingTumour CellsLiver TumourSpleen MobilisationVascular PedicleLewis Lung Carcinoma CellsFirefly Luciferase GeneHemi-spleen ResectionAbdomenBioluminescence ImagingIVIS Whole Body Imaging SystemQuantitative ImagingViable Tumour CellsTumour Cell DeathNecrosisDrug TreatmentMetastatic Tumour-cell SurvivalGrowth MechanismsEvaluation Of Therapeutics

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