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L'imagerie di vivo des parasites Leishmania transgéniques dans un hôte en direct
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1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3Department of Internal Medicine,University of Iowa, 4Department of Molecular Microbiology,Washington University School of Medicine, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Capitoli
- 00:04Titolo
- 00:54Introduction
- 01:37Infection of Mice with Leishmania
- 03:54Bioluminescent Imaging
- 06:18Data Analysis
- 07:04Representative Results
- 09:17Conclusion
Une<em> In vivo</em> Système d'imagerie est utilisée pour générer des mesures quantitatives de l'infection murin avec le protozoaire trypanosomatides<em> Leishmania</em>. C'est une méthode non invasive et non létales pour la détection des parasites exprimant la luciférase dans de nombreux tissus tout au long de chroniques<em> Leishmania</em> Spp. l'infection.
Tags
In Vivo ImagingTransgenic Leishmania ParasitesLive HostLeishmania SpeciesVisceral LeishmaniasisCutaneous LeishmaniasisMouse ModelsParasite BurdensMicroscopyLimiting Dilution AssayQPCR AmplificationIn Vivo Imaging System (IVIS)Bioluminescent SignalLuciferase ExpressionIntradermal InjectionIntravenous InjectionLongitudinal StudiesInter-animal VariationParasite InoculaMultiplication Of ParasitesHigh SensitivityCutaneous Locations
