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In-vivo-Imaging von transgenen Leishmania Parasiten in einer Live-Host
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1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3Department of Internal Medicine,University of Iowa, 4Department of Molecular Microbiology,Washington University School of Medicine, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Capitoli
- 00:04Titolo
- 00:54Introduction
- 01:37Infection of Mice with Leishmania
- 03:54Bioluminescent Imaging
- 06:18Data Analysis
- 07:04Representative Results
- 09:17Conclusion
Ein<em> In vivo</em> Imaging-System wird verwendet, um quantitative Messungen der murinen Infektion mit dem Protozoon Trypanosomatid generieren<em> Leishmania</em>. Dies ist eine nicht-invasive und nicht-tödliche Methode zum Nachweis von Parasiten, die Luciferase in vielen Geweben im Verlauf der chronischen<em> Leishmania</em> Spp. Infektion.
Tags
In Vivo ImagingTransgenic Leishmania ParasitesLive HostLeishmania SpeciesVisceral LeishmaniasisCutaneous LeishmaniasisMouse ModelsParasite BurdensMicroscopyLimiting Dilution AssayQPCR AmplificationIn Vivo Imaging System (IVIS)Bioluminescent SignalLuciferase ExpressionIntradermal InjectionIntravenous InjectionLongitudinal StudiesInter-animal VariationParasite InoculaMultiplication Of ParasitesHigh SensitivityCutaneous Locations
