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High-Throughput Quantification of the Synthesis and Distribution of mtDNA

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Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

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Biochimica

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JoVE Journal Biochimica

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

DOI:

10.3791/200044-v

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03:02 min

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May 05, 2023

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Lukasz S. Borowski², Karolina Kasztelan³, Jolanta Czerwinska-Kostrzewska², Roman J. Szczesny

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Tags

Quantitative Image Analysis MtDNA Levels Gene Expression Transfected HeLa Cells Fluorescence Imaging Background Correction Image Segmentation BrdU Mitochondrial DNA Quantitative Real-time PCR Dideoxycytidine DDC TFAM TWINKLE Helicase POLG Mitochondrial DNA Polymerase Gamma

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