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Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells
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Konstruere design til transfektering af cystein-manipulerede spændingsstyrede Ca2+ kanaler i murin isolerede skeletmuskelfibre
JoVE Journal
Biologia
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JoVE Journal Biologia
Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers

Konstruere design til transfektering af cystein-manipulerede spændingsstyrede Ca2+ kanaler i murin isolerede skeletmuskelfibre

Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

DOI:
10.3791/200065-v

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01:22 min

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June 02, 2023

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, , ,
1Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

Tags

Cysteine-engineered Voltage-gated Ca2+ ChannelsMurine Isolated Skeletal Muscle FibersCaV1.1 CDNA ConstructEGFP-tagged Alpha SubunitCytomegalovirus PromoterSite-directed MutagenesisS4 Membrane Extracellular InterfaceGFP ObservationIonic Current Recording

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