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Spatiotemporal Investigation of ERL1 Dynamics via Confocal Imaging
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Dual-Colored F1 형질전환 Arabidopsis thaliana의 시료 전처리 및 공초점 이미징
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Biologia
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JoVE Journal Biologia
Sample Preparation and Confocal Imaging of Dual-Colored F1 Transgenic Arabidopsis thaliana
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Dual-Colored F1 형질전환 Arabidopsis thaliana의 시료 전처리 및 공초점 이미징

Spatiotemporal Investigation of ERL1 Dynamics via Confocal Imaging

DOI:
10.3791/200076-v

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06:00 min

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May 12, 2023

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, ,
1Department of Biology,Rutgers University, 2Pharmacology and Toxicology Department,University of Arkansas for Medical Sciences

Tags

Sample PreparationConfocal ImagingDual-colored F1 Transgenic Arabidopsis ThalianaTrue LeafAbaxialGlass SlideWaterCover SlipBeam Path514-nanometer LaserYellow Fluorescent Protein (YFP)PMTHYDEmission Band ThresholdsDetection Window530-570 Nanometers555-nanometer LaserRed Fluorescent Protein (RFP)570-630 Nanometers63X 1.2W Core LensSubcellular Membrane Traffic ActivityStomatal Precursor CellsImage SizeScan Head SpeedZoom FactorLine AverageXYT Scan ModeTime SeriesZ-stack Scan ModeMaximum Intensity ProjectionXYZ Scan ModeZ WideZ Step

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