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Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs

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hiPSC-CM의 수축성 측정

JoVE Journal
Biologia

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JoVE Journal Biologia

Contractility Measurements in hiPSC-CMs

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hiPSC-CM의 수축성 측정

Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs

DOI:

10.3791/200096-v

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02:30 min

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May 26, 2023

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Rafeeh Dinani², Emmy Manders^2,3, Michiel Helmes^2,3, Lili Wang, Bjorn C. Knollmann, Diederik W. D. Kuster², Jolanda van der Velden²

¹Division of Physiology, Amsterdam UMC,Vrije University, ²Heart Failure & Arrhythmias,Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology,Vanderbilt School of Medicine

Tags

Contractility HiPSC-CMs Optics-based Measurement System Tyrode’s Solution Climate Control Lid Cell Finder Synchronized Contraction And Relaxation Baseline Measurements Stressor Compound Automatic Measurement

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인간 유도 만능 줄기 세포 유래 심근 세포에서 칼슘 및 수축성 매개변수의 실시간 측정

hiPSC-CM의 수축성 측정

hiPSC-CM의 칼슘 과도 측정

수축성 및 칼슘 과도 측정 데이터 분석

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