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Ricerca sul cancro

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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

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Multiplex immunfluorescensfarging og bildeanalyse

JoVE Journal
Ricerca sul cancro

This content is Free Access.

JoVE Journal Ricerca sul cancro

Multiplex Immunofluorescence Staining and Image Analysis

Multiplex immunfluorescensfarging og bildeanalyse

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

DOI:

10.3791/200119-v

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04:44 min

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June 02, 2023

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Nicolas Huyghe, Elena Benidovskaya, Simon Beyaert, Aurélie Daumerie, Finoula Maestre Osorio, Frank Aboubakar Nana³, Caroline Bouzin, Marc Van den Eynde⁵

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

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Multiplex Immunofluorescence Deparaffinization Endogenous Peroxidase Inhibition Citrate Buffer Microwave Tris-buffered Saline Tween 20 BSA Primary Antibody Poly-HRP Secondary Antibody Tyramide Reagent Pan-Cytokeratin Antibody BisBenzimide Fluorescent Staining Image Analysis

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