/

/

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

/

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

JoVE Journal
Genetica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetica

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

DOI:

10.3791/200140-v

•

02:37 min

•

April 21, 2023

•

Llorenç Rovirosa*¹, Laureano Tomás-Daza*^1,2, Blanca Urmeneta, Alfonso Valencia³, Biola M. Javierre⁴

¹Josep Carreras Leukaemia Research Institute, ²Barcelona Supercomputing Center, ³Catalan Institution for Research and Advanced Studies (ICREA), ⁴Institute for Health Science Research Germans Trias i Pujol

Tags

Keywords: DNA Library Scarce Cell DNA Biotin Pull-down PCR Amplification Library Capture Streptavidin Beads Binding Buffer Wash Buffer DNA Purification Paramagnetic Beads Automated Electrophoresis

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations (Video) | JoVE

Lysis and Digestion of Scarce Cells (Video) | JoVE

Ligation and Decrosslinking of Scarce Cell DNA (Video) | JoVE

Sonication and Biotin Pull-Down of Scarce Cells DNA (Video) | JoVE

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

Read Article