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Purifying a Fibrinolytic Enzyme from Sipunculus nudus via Affinity Chromatography

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Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE

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Biochimica

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JoVE Journal Biochimica

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE

Purifying a Fibrinolytic Enzyme from Sipunculus nudus via Affinity Chromatography

DOI:

10.3791/200167-v

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02:36 min

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June 02, 2023

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Mingqing Tang², Hongjun Lin, Chengjia Hu, Hui Yan²

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Tags

Affinity Chromatography Fibrinolytic Enzymes Protein Purification Arginine Agarose Matrix Lysine Agarose Matrix Tris Hydrochloride Buffer Sodium Chloride Elution Ultracentrifugal Filter

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Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus (Video) | JoVE

Protein Isolation from Sipunculus nudus (Video) | JoVE

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE

Fibrinolytic Activity of the Fibrinolytic Enzyme Purified from Sipunculus nudus (Video) | JoVE

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