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Purifying a Fibrinolytic Enzyme from Sipunculus nudus via Affinity Chromatography

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Affinitätschromatographie-vermittelte Reinigung von fibrinolytischen Enzymen aus Rohproteinextrakten

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts

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Affinitätschromatographie-vermittelte Reinigung von fibrinolytischen Enzymen aus Rohproteinextrakten

Purifying a Fibrinolytic Enzyme from Sipunculus nudus via Affinity Chromatography

DOI:

10.3791/200167-v

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02:36 min

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June 02, 2023

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Mingqing Tang², Hongjun Lin, Chengjia Hu, Hui Yan²

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Tags

Affinity Chromatography Fibrinolytic Enzymes Protein Purification Arginine Agarose Matrix Lysine Agarose Matrix Tris Hydrochloride Buffer Sodium Chloride Elution Ultracentrifugal Filter

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Affinitätsreinigung eines fibrinolytischen Enzyms aus Sipunculus nudus

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Affinitätschromatographie-vermittelte Reinigung von fibrinolytischen Enzymen aus Rohproteinextrakten

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