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在哺乳动物细胞中的DNA双链断裂（DSB）的维修分析

JoVE Journal
Biologia

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JoVE Journal Biologia

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

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在哺乳动物细胞中的DNA双链断裂（DSB）的维修分析

DOI:

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13:10 min

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September 08, 2010

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Andrei Seluanov, Zhiyong Mao, Vera Gorbunova

¹Department of Biology,University of Rochester

Capitoli

00:00Titolo
01:13Introduction
02:22The Reporter Constructs
05:02Integration of the Reporter Constructs and Induction of DSBs
09:18Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
11:34Representative FACS Results of the DSB Repair Assay
12:31Conclusion

Summary

Traduzione automatica

本文介绍了基于GFP的荧光<em>在体内</em>分析，分别量化同源重组和非同源末端在哺乳动物细胞中加入。

Tags

DNA Double-strand Break Repair Mammalian Cells Nonhomologous End Joining NHEJ Homologous Recombination HR Perturbations Premature Aging Tumorigenesis Quantitative Measurement Fluorescent Reporter Constructs GFP Gene I-SceI Endonuclease Induction Of DSBs GFP Positive Cells Flow Cytometry

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