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Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

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Purification de l’ARN poly(A) et conversion du bisulfite pour le séquençage de nouvelle génération

JoVE Journal
Biologia

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JoVE Journal Biologia

Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing

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Purification de l’ARN poly(A) et conversion du bisulfite pour le séquençage de nouvelle génération

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

DOI:

10.3791/200389-v

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05:45 min

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July 07, 2023

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Szu-Ying Chen², Po-Hsien Huang²

¹Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University

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Keywords: Poly(A) RNA Purification Bisulfite Conversion Next-generation Sequencing Total RNA Oligo(dT) Beads Lysis Buffer Wash Buffer Tris-HCl RNA Elution Magnetic Separation RNA Sample Preparation RNA Enrichment

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Enrichissement de la bibliothèque d’ARNm et de bisulfite-ARNm pour le séquençage de nouvelle génération

Purification de l’ARN poly(A) et conversion du bisulfite pour le séquençage de nouvelle génération

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