/

/

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

/

Poly(A)-RNA-Aufreinigung und Bisulfit-Umwandlung für die Sequenzierung der nächsten Generation

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing

Please note that all translations are automatically generated. Click here for the English version.

Poly(A)-RNA-Aufreinigung und Bisulfit-Umwandlung für die Sequenzierung der nächsten Generation

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

DOI:

10.3791/200389-v

•

05:45 min

•

July 07, 2023

•

Szu-Ying Chen², Po-Hsien Huang²

¹Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University

Tags

Keywords: Poly(A) RNA Purification Bisulfite Conversion Next-generation Sequencing Total RNA Oligo(dT) Beads Lysis Buffer Wash Buffer Tris-HCl RNA Elution Magnetic Separation RNA Sample Preparation RNA Enrichment

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Anreicherung von mRNA- und Bisulfit-mRNA-Bibliotheksvorbereitung für Next-Generation-Sequencing

Poly(A)-RNA-Aufreinigung und Bisulfit-Umwandlung für die Sequenzierung der nächsten Generation

Read Article