/
/
/
用于 不动杆菌 生物膜形成的细菌接种物的制备
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Tags
Bacterial Inoculum Preparation For Acinetobacter Biofilm Formation:
1. Inoculate A Blood Agar Plate With 2-10 μL Of The Bacterial Strain Using A Sterile Pipette Tip. Streak The Agar SurfaceThinning It Out With Intermittent Flaming. Incubate The Plate At 30°C For 20-24 Hours.
2. Select A Single Colony From The Agar Plate And Inoculate 5 ML Of Sterile BHI Broth. Incubate The Inoculated Broth In A Shaking Incubator At 30°C150 Rpm For 20-24 Hours.
3. Transfer 1 ML Of The Culture To A Sterile Microcentrifuge Tube And Centrifuge At 6000 G For 10 Minutes At Room Temperature. Discard The Supernatant.
4. Add 1 ML Of Sterile Phosphate-buffered Saline (PBS) To The Pellet And Resuspend Using A Vortex. Centrifuge At 6000 G For 10 Minutes At Room Temperature And Discard The Supernatant.
5. Resuspend The Resulting Pellet In Sterile10-fold Diluted BHI Broth Using A Vortex. Ensure The Optical Density Is Around 0.1 At 600 Nm
