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To-trins udvidelse af humane mesenkymale stamceller fra 5-liters til 15-liters bioreaktorer og automatiseret celleformulering fra en 15-liters bioreaktor
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor
To-trins udvidelse af humane mesenkymale stamceller fra 5-liters til 15-liters bioreaktorer og automatiseret celleformulering fra en 15-liters bioreaktor
DOI:
10.3791/200420-v
•
06:10 min
•
July 07, 2023
•
Yuyang Chen*
1
,
Huanye Xu*
2
,
Yuanyuan Zhang*
2
,
Leilei Guo
,
Miaomiao Lan
,
Yanping Yang
,
Wei Liu
,
Xiaojun Yan
,
Yanan Du
1
Department of Biomedical Engineering, School of Medicine
,
Tsinghua University
,
2
Beijing CytoNiche Biotechnology Co. Ltd.
Tags
Mesenchymal Stem Cells
Bioreactor
Cell Expansion
Automated Cell Formulation
Cell Harvest
Cell Processing
Microcaririer
Serum-free Medium
Cell Inoculation
Medium Supplement
Medium Exchange
Cell Sampling
Cell Harvest
Cell Suspension
Cell Washing
Cell Culture
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