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Bacterial Metabolic Inactivation in Caenorhabditis elegans Using Paraformaldehyde

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Bacteriële inoculatie en paraformaldehyde-inactivatie voor Caenorhabditis elegans-onderzoek

JoVE Journal
Biologia

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JoVE Journal Biologia

Bacterial Inoculation and Paraformaldehyde Inactivation for Caenorhabditis elegans Ricerca

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Bacteriële inoculatie en paraformaldehyde-inactivatie voor Caenorhabditis elegans-onderzoek

Bacterial Metabolic Inactivation in Caenorhabditis elegans Using Paraformaldehyde

DOI:

10.3791/200464-v

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06:10 min

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July 28, 2023

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Safa Beydoun*¹, Elizabeth S. Kitto*¹, Emily Wang, Shijiao Huang, Scott F. Leiser²

¹Molecular and Integrative Physiology Department,University of Michigan, Ann Arbor, ²Department of Internal Medicine,University of Michigan, Ann Arbor

Tags

Bacterial Inoculation Paraformaldehyde Inactivation Caenorhabditis Elegans Luria Broth Shaker Incubator Optical Density Aliquot Serological Pipette Paraformaldehyde Concentration Centrifugation Resuspension Lifespan Assay Bacterial Growth Contamination Check Respirometry

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Methodologie om bacteriën metabolisch te inactiveren voor Caenorhabditis elegans-onderzoek

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