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Biologia dello sviluppo

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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

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从诱导多能干细胞悬浮培养物中产生肾脏类器官

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Generating Kidney Organoids from Induced Pluripotent Stem Cell Suspension Culture

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从诱导多能干细胞悬浮培养物中产生肾脏类器官

Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

DOI:

10.3791/200500-v

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02:36 min

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September 01, 2023

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Langping Gao*^1,2, Yue Wang*³, Gang Wang, Hangdi Wu, Qingtao Yan, Jingjing Wang, Fei Liu, Haidong Fu, Wei Li, Lidan Hu, Jianhua Mao²

¹Department of Nephrology, The Children’s Hospital,Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital,Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital,Zhejiang University School of Medicine, National Clinical Research Center for Child Health

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Keywords: Kidney Organoids Induced Pluripotent Stem Cells Suspension Culture Cell Detachment Stage III Medium Low-adhesion Plate Orbital Shaker Stage IV Medium Tubular Structures CD31 Endothelial Cells Dextran Uptake

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