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İndüklenmiş Pluripotent Kök Hücre Süspansiyon Kültüründen Böbrek Organoidlerinin Üretilmesi

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Developmental Biology

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JoVE Journal Developmental Biology

Generating Kidney Organoids from Induced Pluripotent Stem Cell Suspension Culture

Please note that all translations are automatically generated. Click here for the English version.

İndüklenmiş Pluripotent Kök Hücre Süspansiyon Kültüründen Böbrek Organoidlerinin Üretilmesi

DOI:

10.3791/200500-v

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02:36 min

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September 01, 2023

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Langping Gao*^1,2, Yue Wang*³, Gang Wang, Hangdi Wu, Qingtao Yan, Jingjing Wang, Fei Liu, Haidong Fu, Wei Li, Lidan Hu, Jianhua Mao²

¹Department of Nephrology, The Children’s Hospital,Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital,Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital,Zhejiang University School of Medicine, National Clinical Research Center for Child Health

Tags

Kidney Organoids Induced Pluripotent Stem Cells Suspension Culture Cell Detachment Stage III Medium Low-adhesion Plate Orbital Shaker Stage IV Medium Tubular Structures CD31 Endothelial Cells Dextran Uptake

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