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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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एक माउस रीढ़ की हड्डी से अलग माइक्रोग्लियल कोशिकाओं के लिबरेज़-आधारित एंजाइमेटिक शुद्धि

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Neuroscienze

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JoVE Journal Neuroscienze

Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord

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एक माउस रीढ़ की हड्डी से अलग माइक्रोग्लियल कोशिकाओं के लिबरेज़-आधारित एंजाइमेटिक शुद्धि

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

DOI:

10.3791/200616-v

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02:42 min

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September 22, 2023

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Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children’s Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

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Keywords: Microglia Isolation Spinal Cord Enzymatic Digestion Liberase Density Gradient Cell Purification FACS Analysis

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माउस स्पाइनल कॉर्ड माइक्रोग्लिया का तेजी से और कुशल संवर्धन

एक माउस रीढ़ की हड्डी से अलग माइक्रोग्लियल कोशिकाओं के लिबरेज़-आधारित एंजाइमेटिक शुद्धि

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