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Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies
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Substrat-afkobler-inhibitor-titrering til mitokondriel respirationsanalyse i Drosophila melanogaster
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JoVE Journal Biochimica
Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster

Substrat-afkobler-inhibitor-titrering til mitokondriel respirationsanalyse i Drosophila melanogaster

Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies

DOI:
10.3791/200718-v

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05:12 min

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November 10, 2023

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1Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences,Federal University of Santa Maria, 2Graduate Program in Biological Sciences: Toxicological Biochemistry,Federal University of Santa Maria

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Keywords: Mitochondrial RespirationDrosophila MelanogasterSubstrateUncouplerInhibitorTitrationPolarographic Oxygen SensorsMiR05 BufferHigh-resolution RespirometryDigitoninPyruvateMalateProlineADPSuccinateOligomycinFCCPRotenoneMalonateAntimycinOXPHOS CIOXPHOS CIIETS CIETS CIIATP SynthesisOXPHOS/LEAK Ratio

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