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Assessing Genomic Alteration in Non-Squamous Non-Small Cell Lung Cancer Patients

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Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API) (Video) | JoVE

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Biologia

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JoVE Journal Biologia

Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API)

Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API) (Video) | JoVE

Assessing Genomic Alteration in Non-Squamous Non-Small Cell Lung Cancer Patients

DOI:

10.3791/200724-v

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02:34 min

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September 08, 2023

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Christophe Bontoux^2,3,4,5, Virginie Lespinet-Fabre^2,3,4, Olivier Bordone^2,3,4, Virginie Tanga^2,3,4, Maryline Allegra^2,3,4, Myriam Salah^2,3,4, Salomé Lalvée^2,3,4, Samantha Goffinet^2,3,4, Jonathan Benzaquen^4,5,6, Charles-Hugo Marquette^4,5,6, Marius Ilié^2,3,4,5, Véronique Hofman^2,3,4,5, Paul Hofman^2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d’Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d’Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur

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Keywords: FFPE DNA RNA Sequencing Automated Purification Instrument API Integrated Genomic Viewer Protease Digest Centrifugation DNA Digestion Nucleic Acid Purification Plate

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