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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation
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One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)
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Biologia
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JoVE Journal Biologia
One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)

One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)

Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

DOI:
10.3791/200810-v

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03:01 min

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April 26, 2024

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, , , , ,
1Department of Cardiovascular Surgery, West China Hospital,Sichuan University, 2Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, 3West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, 4Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

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Keywords: Rat Neutrophil IsolationNeutrophil Extracellular Traps (NETs)Bone Marrow HarvestDensity Gradient CentrifugationOne-step MethodTwo-step MethodRPMI MediaRed Blood Cell Lysis BufferPercoll ReagentHBSSCell ViabilityHemocytometerTrypan Blue Staining

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