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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

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用于收获中性粒细胞胞外诱捕器（NET）的大鼠中性粒细胞分离的一步法和两步法

JoVE Journal
Biologia

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JoVE Journal Biologia

One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)

Please note that all translations are automatically generated. Click here for the English version.

用于收获中性粒细胞胞外诱捕器（NET）的大鼠中性粒细胞分离的一步法和两步法

Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

DOI:

10.3791/200810-v

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03:01 min

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April 26, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang³, Zhenghua Xiao⁴, Li He⁴, Chaoyi Qin⁴

¹Department of Cardiovascular Surgery, West China Hospital,Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

Tags

Rat Neutrophil Isolation Neutrophil Extracellular Traps (NETs)Bone Marrow Harvest Density Gradient Centrifugation One-step Method Two-step Method RPMI Media Red Blood Cell Lysis Buffer Percoll Reagent HBSS Cell Viability Hemocytometer Trypan Blue Staining

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