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Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models
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Трансфекция и трансдукция мышиных Т-клеток для генерации CAR-T-клеток
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Bioingegneria
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JoVE Journal Bioingegneria
Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation
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Трансфекция и трансдукция мышиных Т-клеток для генерации CAR-T-клеток

Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models

DOI:
10.3791/200881-v

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05:34 min

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February 02, 2024

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, , , ,
1Department of Biomedical Engineering,Columbia University, 2Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons,Columbia University, 3Herbert Irving Comprehensive Cancer Center,Columbia University, 4Data Science Institute,Columbia University

Tags

Murine T-cellsCAR-T Cell GenerationTransfectionTransductionPhoenix Eco PlateViral SupernatantSplenocytesCD3 Positive T-cellsNegative SelectionT-cell ActivationHuman Fibronectin Transduction Enhancer

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