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Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models

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Transfektion och transduktion av murina T-celler för CAR-T-cellgenerering

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation

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Transfektion och transduktion av murina T-celler för CAR-T-cellgenerering

Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models

DOI:

10.3791/200881-v

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05:34 min

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February 02, 2024

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Rosa L. Vincent, Fangda Li, Edward R. Ballister, Nicholas Arpaia³, Tal Danino^3,4

¹Department of Biomedical Engineering,Columbia University, ²Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons,Columbia University, ³Herbert Irving Comprehensive Cancer Center,Columbia University, ⁴Data Science Institute,Columbia University

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Murine T-cells CAR-T Cell Generation Transfection Transduction Phoenix Eco Plate Viral Supernatant Splenocytes CD3 Positive T-cells Negative Selection T-cell Activation Human Fibronectin Transduction Enhancer

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