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Transcriptome Profiling for High-Throughput Drug Screening

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Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE

JoVE Journal
Biologia

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JoVE Journal Biologia

Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing

Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE

Transcriptome Profiling for High-Throughput Drug Screening

DOI:

10.3791/200934-v

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01:56 min

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February 23, 2024

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Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

Tags

PBMC Cell Lysate MRNA Sequencing Drug Screening Cell Culture Cell Counting Trypan Blue Cell Suspension Cell Viability 96-well Plate Cell Lysis PBS Lysis Buffer PCR Plate

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