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Transcriptome Profiling for High-Throughput Drug Screening

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Geneesmiddelscreening met behulp van perifere mononucleaire bloedcellen (PBMC's) en cellysaatpreparaat voor mRNA-sequencing

JoVE Journal
Biologia

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JoVE Journal Biologia

Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing

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Geneesmiddelscreening met behulp van perifere mononucleaire bloedcellen (PBMC's) en cellysaatpreparaat voor mRNA-sequencing

Transcriptome Profiling for High-Throughput Drug Screening

DOI:

10.3791/200934-v

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01:56 min

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February 23, 2024

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Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

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PBMC Cell Lysate MRNA Sequencing Drug Screening Cell Culture Cell Counting Trypan Blue Cell Suspension Cell Viability 96-well Plate Cell Lysis PBS Lysis Buffer PCR Plate

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