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Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

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התמיינות תאי גזע פלוריפוטנטיים אנושיים במבחנה לתאי לבלב מייצרי אינסולין

JoVE Journal
Biologia

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JoVE Journal Biologia

In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells

Please note that all translations are automatically generated. Click here for the English version.

התמיינות תאי גזע פלוריפוטנטיים אנושיים במבחנה לתאי לבלב מייצרי אינסולין

Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

DOI:

10.3791/200998-v

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05:06 min

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February 02, 2024

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Ines Cherkaoui, Qian Du, Dieter Egli, Shivani Misra³, Guy A. Rutter^4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

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Keywords: In Vitro Human Pluripotent Stem Cells Insulin-producing Pancreatic Cells Cell Passage Coating Solution Dissociation Solution Trypan Blue Hemocytometer Rock Inhibitor Basal Medium Supplements Washing Medium Day One Medium Pancreatic Progenitor Medium Dissociation Buffer Microwells Anti-Adherence Rinsing Solution

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Optimized Protocol for Generating Functional Pancreatic Insulin-secreting Cells from Human Pluripotent Stem Cells

התמיינות תאי גזע פלוריפוטנטיים אנושיים במבחנה לתאי לבלב מייצרי אינסולין

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