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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

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Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

JoVE Journal
Biologia

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JoVE Journal Biologia

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

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Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/201003-v

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03:07 min

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June 23, 2023

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Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

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Keywords: Immunofluorescence Staining Confocal Microscopy CRISPR/Cas9 CENP-E HeLa Cells Cell Fixation Cell Permeabilization Primary Antibody Secondary Antibody DAPI Chromosome Misalignment Metaphase Interphase Prophase Anaphase Telophase

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