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Construction of a 3D Intestinal Mucosa Model with Paraffin Embedding

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Preparation of a Basic 3D Reconstructed Intestinal Mucosa Model for Microscopic Evaluation of Fixed Intestinal Equivalents

JoVE Journal
Biologia

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JoVE Journal Biologia

Preparation of a Basic 3D Reconstructed Intestinal Mucosa Model for Microscopic Evaluation of Fixed Intestinal Equivalents

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Preparation of a Basic 3D Reconstructed Intestinal Mucosa Model for Microscopic Evaluation of Fixed Intestinal Equivalents

Construction of a 3D Intestinal Mucosa Model with Paraffin Embedding

DOI:

10.3791/201006-v

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03:37 min

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September 01, 2023

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Francesca Truzzi, Silvia Dilloo, Xinyue Chang, Anne Whittaker, Eros D’Amen, Giovanni Dinelli

¹Department of Agricultural and Food Sciences,Alma Mater Studiorum-University of Bologna

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3D Reconstructed Intestinal Mucosa Model Microscopic Evaluation Fixed Intestinal Equivalents HBSS Cell-free Collagen Solution DMEM L-929 Fibroblasts Trypsin EDTA U-937 Monocytes Caco-2 Cells PBS Cell Suspension Collagen Cell Solution Lamina Propria

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