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Profiling the Bacterial Community in Grapes Using Amplicon Metagenomics
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High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity
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Biologia
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JoVE Journal Biologia
High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity
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High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity

Profiling the Bacterial Community in Grapes Using Amplicon Metagenomics

DOI:
10.3791/201076-v

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04:21 min

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December 01, 2023

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, , , ,
1A.R. Smith Department of Chemistry and Fermentation Sciences,Appalachian State University, 2Faculdade de Ciências Farmacêuticas de Ribeirão Preto,Universidade de São Paulo

Tags

Keywords: 16S RRNA GeneV4 Hypervariable RegionPCRSequencingAdapter LigationMagnetic BeadsDNA ConcentrationFluorimeterFASTQ FilesMapping FileQIIME2DADA2Naive Bayes ClassifierSilva DatabaseOTUTaxonomic AssignmentMicrobiomeDB

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