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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

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Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)

JoVE Journal
Biologia

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JoVE Journal Biologia

Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)

Ultrathin Sectioning for Epon Post-Embedding Correlative Light and Electron Microscopy (CLEM)

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

DOI:

10.3791/201225-v

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00:53 min

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January 12, 2024

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Shuyuan Wang*¹, Haiyan Xiong*^1,2, Qiyuan Chang*^1,3, Xudong Zhuang*^4,5,6, Yaochen Wu³, Xinrui Wang^5,6, Congxian Wu, Zhifei Fu^7,8

¹Public Technology Service Center,Fujian Medical University, ²The School of Pharmacy,Fujian Medical University, ³The School of Basic Medical Sciences,Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁵Medical Research Center,Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience,Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

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Keywords: Ultrathin Sectioning Epon Post-embedding Correlative Light And Electron Microscopy CLEM Diamond Knife Ultra Microtome 100 Nanometers Water Bath Gold Nanoparticle Coated Cover Glass Sample Loop Marker Pen

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